Journal: The Journal of Neuroscience

Article Title: Conditional Deletion of α-CaMKII Impairs Integration of Adult-Generated Granule Cells into Dentate Gyrus Circuits and Hippocampus-Dependent Learning

doi: 10.1523/JNEUROSCI.0652-14.2014

Figure Lengend Snippet: Expression of α-CaMKII in dentate granule cells. Colocalization of α-CaMKII (green) expression with (A) nestin (red), (B) Ki67 (red), (C) DCX (red), (D) calretinin (red), (E) NeuN (red), and (F) calbindin (red) in the dentate gyrus of 4- to 5-week-old mice. G–I, Time course of α-CaMKII expression. WT mice were injected with BrdU and killed 1, 3, or 6 weeks later. Examples of BrdU+ cells at (G) 1 week and (H) 6 weeks. I, Percentage of BrdU+ cells coexpressing α-CaMKII. Scale bar, 10 μm. Mo, Molecular layer; GCL, granule cell layer.

Article Snippet: Granule cell layer volume was estimated in α-CaMKII +/+ ( n = 7) and α-CaMKII-iKO ( n = 7) mice from 10 hippocampal slices per mouse (collected every sixth section) using the Cavalieri volume estimation method (magnification: 20×; grid spacing: 40 μm; Cavalieri estimator module in Stereo Investigator, MBF Bioscience).

Techniques: Expressing, Injection

Journal: The Journal of Neuroscience

Article Title: Conditional Deletion of α-CaMKII Impairs Integration of Adult-Generated Granule Cells into Dentate Gyrus Circuits and Hippocampus-Dependent Learning

doi: 10.1523/JNEUROSCI.0652-14.2014

Figure Lengend Snippet: α-CaMKII expression pattern in DGCs

Article Snippet: Granule cell layer volume was estimated in α-CaMKII +/+ ( n = 7) and α-CaMKII-iKO ( n = 7) mice from 10 hippocampal slices per mouse (collected every sixth section) using the Cavalieri volume estimation method (magnification: 20×; grid spacing: 40 μm; Cavalieri estimator module in Stereo Investigator, MBF Bioscience).

Techniques: Expressing, Marker

Journal: The Journal of Neuroscience

Article Title: Conditional Deletion of α-CaMKII Impairs Integration of Adult-Generated Granule Cells into Dentate Gyrus Circuits and Hippocampus-Dependent Learning

doi: 10.1523/JNEUROSCI.0652-14.2014

Figure Lengend Snippet: Conditional deletion of α-CaMKII impairs morphological development of newly generated dentate granule cells. A, Schematic of the strategy for conditional deletion of α-CaMKII from NPCs. In nestin-CreERT2+/α-CaMKIIf/f mice (α-CaMKII-iKO), TAM administration leads to excision of α-CaMKII exon 2 flanked sequences and deletion of α-CaMKII in neural progenitor cells and their progeny. B, Eight weeks after TAM treatment, the vast majority of recombined cells (LacZ+; red) lacked α-CaMKII (green). Scale bars, 10 μm. C, Retrovirally labeled dentate granule cells in control (left; α-CaMKII f/f) and α-CaMKII-iKO (right) mice, 8 weeks after infection. In α-CaMKII-iKO (right) mice, GFP-labeled dentate granule cells dendrites had more (D) bifurcations and (E) branches. Eight weeks after TAM treatment, expression of (F) nestin (green), (G) DCX (green), (H) NeuN (green), and (I) calbindin (green) in recombined (LacZ+; red) cells was similar in control (top; α-CaMKIIf/f) and α-CaMKII-iKO (bottom) mice. J, Left, Eight weeks after TAM treatment, expression of zif268 (green) in recombined (LacZ+; red) in control (α-CaMKIIf/f) and α-CaMKII-iKO mice. Right, Contextual fear conditioning (trained) induced zif268 expression in recombined (LacZ+) cells in α-CaMKII-iKO mice compared with home cage (HC) controls. Scale bar, 10 μm. *p < 0.05.

Article Snippet: Granule cell layer volume was estimated in α-CaMKII +/+ ( n = 7) and α-CaMKII-iKO ( n = 7) mice from 10 hippocampal slices per mouse (collected every sixth section) using the Cavalieri volume estimation method (magnification: 20×; grid spacing: 40 μm; Cavalieri estimator module in Stereo Investigator, MBF Bioscience).

Techniques: Generated, Labeling, Control, Infection, Expressing

Journal: The Journal of Neuroscience

Article Title: Conditional Deletion of α-CaMKII Impairs Integration of Adult-Generated Granule Cells into Dentate Gyrus Circuits and Hippocampus-Dependent Learning

doi: 10.1523/JNEUROSCI.0652-14.2014

Figure Lengend Snippet: Conditional deletion of α-CaMKII impairs integration and survival of newly generated dentate granule cells. A, Retrovirally labeled granule cell dendritic segments (green) in control (left; α-CaMKIIf/f) and α-CaMKII-iKO (right) mice, 8 weeks after infection. Spine density was similar in α-CaMKII-iKO (closed bars) compared with α-CaMKIIf/f (open bars) mice. Scale bar, 1 μm. B, Retrovirally labeled granule cell spine terminals (green) and VGlut1+ puncta (red). Examples of nonjuxtaposed (top) and juxtaposed (bottom) spine terminals. In α-CaMKII-iKO mice, there was a reduction in the proportion of spines in direct apposition to VGlut1+ puncta. Scale bar, 0.5 μm. C, α-CaMKII+/+ and α-CaMKII-iKO mice were injected with TAM and subsequently injected with BrdU to label newly generated neurons. BrdU was injected twice a day for 3 d, 24 h after the last TAM injection. D, Eight weeks later, the number of BrdU+ cells (red) was reduced in α-CaMKII-iKO (open bars) compared with α-CaMKII+/+ (closed bars) mice. Examples of BrdU+ cells at higher magnification are shown from the insets (right). Scale bars: left, 100 μm; right, 10 μm. *p < 0.05.

Article Snippet: Granule cell layer volume was estimated in α-CaMKII +/+ ( n = 7) and α-CaMKII-iKO ( n = 7) mice from 10 hippocampal slices per mouse (collected every sixth section) using the Cavalieri volume estimation method (magnification: 20×; grid spacing: 40 μm; Cavalieri estimator module in Stereo Investigator, MBF Bioscience).

Techniques: Generated, Labeling, Control, Infection, Injection

Journal: The Journal of Neuroscience

Article Title: Conditional Deletion of α-CaMKII Impairs Integration of Adult-Generated Granule Cells into Dentate Gyrus Circuits and Hippocampus-Dependent Learning

doi: 10.1523/JNEUROSCI.0652-14.2014

Figure Lengend Snippet: Conditional deletion of α-CaMKII impairs contextual fear learning. A, Experimental design. Mice underwent contextual fear conditioning (1 shock) 8 weeks after TAM administration and were tested 1 d later. During training, (B) shock reactivity and (C) postshock freezing were similar in control (α-CaMKII f/f; closed bars) and α-CaMKII-iKO (open bars) mice. During testing, α-CaMKII-iKO showed reduced freezing levels (D, minute-by-minute freezing; E, total freezing in the 5 min test). F, Experimental design. Mice underwent contextual fear conditioning (3 shocks) 8 weeks after TAM administration and were tested 1 d later. During training, (G) shock reactivity and (H) postshock freezing were similar in control (α-CaMKIIf/f; closed bars) and α-CaMKII-iKO (open bars) mice. During testing, α-CaMKII f/f and α-CaMKII-iKO mice exhibited equivalent freezing levels (I, minute-by-minute freezing; J, total freezing in the 5 min test). In the open field, α-CaMKII f/f (closed bars) and α-CaMKII-iKO (open bars) mice (K) covered equivalent total distance and (L) spent equivalent time in different regions of the arena. In the forced swim test, (M) latency to become immobile and (N) total time spent immobile were equivalent in α-CaMKIIf/f (closed bars) and α-CaMKII-iKO (open bars) mice. *p < 0.05.

Article Snippet: Granule cell layer volume was estimated in α-CaMKII +/+ ( n = 7) and α-CaMKII-iKO ( n = 7) mice from 10 hippocampal slices per mouse (collected every sixth section) using the Cavalieri volume estimation method (magnification: 20×; grid spacing: 40 μm; Cavalieri estimator module in Stereo Investigator, MBF Bioscience).

Techniques: Control